?
Welcome to Guangzhou IGE Biotechnology Co., Ltd. Website !
Main Page > Services > Next-generation sequencing > Small RNA sequencing

 Small RNA sequencing



Service introduction




        Small RNAs (sRNA) are important regulators of life activities, playing important roles in gene expression regulation, individual development, metabolism, and the occurrence of diseases. The Illumina sequencing platform can perform deep sequencing of all sRNA in the sample to achieve qualitative and quantitative research purposes. Each sample can obtain more than 10 million sRNA sequencing sequences. Through massive parallel sequencing, it can discover, identify, and quantify the small RNA map at the whole genome level of any species, the excavation of new mRNA molecules, the prediction of target genes, identification, and differential expression analysis between samples.

 

Technical procedures




       圖片6

 

Sample requirements




 

實驗類型

RNA樣品總量

樣品濃度和純度

 樣品質量

樣品保存

樣品運輸

Small RNA測序

一次性提供至少3 μg高質量的總 RNA

樣品濃度 >100 ng/μl,OD260/280 介于 1.8-2.0 之間,無肉眼可見污染 

使用 Agilent Bioanalyzer 儀器檢測,則獲得的總的 RNA 28S:18S >1,RIN>7.5

請選擇乙醇、DEPC 水保存樣品,并在樣品信息單中注明 

樣品請置于 1.5 ml 管中,并使用封口膜封好,放置足夠的干冰運輸

 

Experiment cycle and sequencing strategy



 

實驗類型

測序策略

 數據量

實驗周期

Small RNA測序

HiseqSE50 

10M

樣品質檢合格后,30-50 個工作日

 


HotServices
020-89053723
Guangzhou IGE Biotechnology Co., Ltd.
Email: marketing@igebio.com
QQ: 1787730693
Address: Building G1-903, South China New Materials Innovation Park, #31 Kefeng Road, Huangpu Dist. Guangzhou, Guangdong prov. China 510663

Copyright ? Guangzhou IGE Biotechnology Co., Ltd. 粵ICP備2021146096號
Contact Us
  • Contact Us